Optional, maximum resolution limited by available bandwidthĢ0 pins for external connectors on desktops, notebooks, graphics cards, monitors, etc. Optional 1–8 channels, 16 or 24-bit linear PCM 32–192 kHz sampling rate maximum bitrate 36,864 kbit/s (4,608 kB/s) We show how by combining the Connectivity Map (CMAP) with ChEA, we can rank pairs of compounds to be used to target specific transcription factor activity in cancer cells.ĪVAILABILITY: The ChEA software and ChIP-X database is freely available online at. To demonstrate the utility of ChEA we present three case studies. The ChEA database allowed us to reconstruct an initial network of transcription factors connected based on shared overlapping targets and binding site proximity. With ChEA, users can input lists of mammalian gene symbols for which the program computes over-representation of transcription factor targets from the ChIP-X database. The system is delivered as a web-based interactive application called ChIP Enrichment Analysis (ChEA). We used the database to analyze mRNA expression data where we perform gene-list enrichment analysis using the ChIP-X database as the prior biological knowledge gene-list library. The database contains 189,933 interactions, manually extracted from 87 publications, describing the binding of 92 transcription factors to 31,932 target genes. RESULTS: In order to integrate data from such studies and utilize it for further biological discovery, we collected interactions from such experiments to construct a mammalian ChIP-X database. Such experiments provide hundreds to thousands of potential binding sites for a given transcription factor in proximity to gene coding regions. MOTIVATION: Experiments such as ChIP-chip, ChIP-seq, ChIP-PET and DamID (the four methods referred herein as ChIP-X) are used to profile the binding of transcription factors to DNA at a genome-wide scale.
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